A single-nucleotide polymorphism of GRIN1 in heroin and methamphetamine addicts at a rehabilitation sanatorium in Markazi province, Iran

AUTHORS

Ahmad Hamta 1 , * , Maryam sahraei 1

1 Dept. of Biology, Faculty of Science, Arak University, Arak, Iran

How to Cite: Hamta A, sahraei M. A single-nucleotide polymorphism of GRIN1 in heroin and methamphetamine addicts at a rehabilitation sanatorium in Markazi province, Iran, J Kermanshah Univ Med Sci. 2017 ; 20(4):e69665. doi: 10.22110/jkums.v20i4.3207.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 20 (4); e69665
Published Online: March 19, 2017
Article Type: Original Article
Received: September 27, 2016
Accepted: January 15, 2017
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Abstract

Introduction: Using addictive drugs can change the amount of neurotransmitters, especially dopamine and glutamate. Glutamate has been known to trigger the relapse and tendency toward addictive drugs. The glutamate receptor ionotropic NMDA type subunit 1 (GRIN1) contains the single- nucleotide polymorphism C1001G (rs11146020) and encodes N-methyl-D-aspartic acid (NDMA) receptor subunit 1 (NR1). The present study was conducted to investigate the relationship between the rs11146020 polymorphism in GRIN1 and addiction to heroin and methamphetamine.

Methods: The present case-control study recruited 90 male heroin and methamphetamine addicts treated with methadone and 100 healthy men. Genomic DNA was extracted from peripheral blood using Iraizol kits. Four pairs of specific primers were designed using AlleleID 7.5, and the T-ARMS PCR was optimized.

Results: The genotype distribution of GG, GC and CC was respectively found to be 66%, 31% and 3% in the control group and 58%, 31% and 11% in the patient group. The statistical analysis suggested no significant differences between these two groups.

Conclusion: No significant relationships were observed between the C1001G polymorphism in GRIN1 and addiction to heroin and methamphetamine.

Keywords

Addiction GRIN1 single-nucleotide polymorphism T-ARMS PCR

© 2017, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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