The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis

AUTHORS

Ali Gorgeen Karaji 1 , * , Firouzeh Abdi 2 , Mansour Rezaei 3

1 Dept. of Immonology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Central Labratory, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Dept. of Biostatistics and Epidemiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

How to Cite: Gorgeen Karaji A , Abdi F , Rezaei M . The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis, J Kermanshah Univ Med Sci. 2011 ; 15(1):e69718.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 15 (1); e69718
Published Online: May 19, 2011
Article Type: Original Article
Received: January 29, 2010
Accepted: June 06, 2010

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Abstract

Background: Rose Bengal is a common test for serologic diagnosis of brucellosis. The aim of this study was to evaluate the agreement rate between modified rose Bengal, rose Bengal and rapid Wright tests for detection of antibody positive serum samples. 

Methods: Cross section study was designed. 941 serum sample of patients referred to the central medical laboratory of Kermanshah, were evaluated by rose Bengal, modified rose Bengal and rapid Wright, using convenient sampling method. Positive samples were confirmed and their titers were defined by Wright agglutination test.

Results: The number of positive serum samples with modified rose Bengal was two fold higher than conventional rose Bengal test (P<0.001), which included all positive samples with rose Bengal. On the other hand, although the number of positive diagnosed serum samples with modified rose Bengal were nearly two times more than Wright test (P<0.001), but it did not include all positive samples detected by Wright test. All three tests showed acceptable agreement rate (k≥0.583).

Conclusion: Results of this study confirmed that modified rose Bengal able to detect more positive serum sample than conventional rose Bengal and it would be substituted rose Bengal. However, the rapid Wright test should be used aside with modified rose Bengal, because it could not cover all positive samples detected by rapid Wright test.

Keywords

rose Bengal modified rose Bengal rapid Wright

© 2011, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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