The effect of β-ionone on telomerase activity in the human leukemia cell line K562

AUTHORS

Zohreh Faezizadeh 1 , * , Amir Gharib 2 , Masoud Goudarzi 3

AUTHORS INFORMATION

1 Department of Laboratory Sciences, Faculty of Paramedical Sciences, Borujerd Branch, Islamic Azad University, Borujerd

2 Department of Laboratory Sciences, Faculty of Paramedical Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

3 Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 19 (3); e69871
Published Online: July 19, 2015
Article Type: Original Article
Received: January 10, 2015
Accepted: June 02, 2015
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Abstract

Background: Telomerase is highly activated in most human cancer cells, therefore, its inhibition has been proposed as a novel and promising strategy for cancer therapy. Many plant-derived anticancer agents act through inhibition of telomerase activity and induction of apoptosis. β-ionone, a carotenoid compound isolated from Roseaceae, has been reported to possess anticancer properties. The present study was undertaken to examine the mechanism of β-ionone-induced apoptosis in human leukemia cell line K562 with special emphasis on its role in telomerase inhibition.

Method: In this study the anti-proliferation effect of β-ionone on K562 cells was evaluated by MTT assay. Apoptosis rate was detected by Hoechst staining and flow cytometry analysis. Telomerase activity was measured by (TRAP) ELISA assay.

Results: Exposure of K562 cells to β-ionone caused a dose-dependent decrease in proliferation. Flow cytometry analysis and Hoechst staining showed that percentage of apoptotic cells markedly increased with an increase in β-ionone concentration. Compared to control cells, treatment of K562 cells with β-ionone resulted in a significant decrease of telomerase activity. Moreover, a positive correlation was detected between telomerase inhibition and apoptosis induction in the treated K562 cells. 

Conclusion: Based on these results, β-ionone is an appropriate candidate for inhibiting telomerase activity in K562 cells. Therefore, it may be utilized as a novel drug against some leukemia cell lines.

Keywords

human leukemia β-ionone telomerase inhibitors apoptosis

© 2015, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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