Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family

AUTHORS

Sina Pejman 1 , Majid Mahdavi 1 , * , Saeed Balalaei 2

AUTHORS INFORMATION

1 Dept. of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

2 Dept. of Organic Chemistry, K. N. Toosi University of Tech, Tehran, Iran

How to Cite: Pejman S, Mahdavi M, Balalaei S. Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family , J Kermanshah Univ Med Sci. 2013 ; 17(7):e74458.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 17 (7); e74458
Published Online: October 29, 2013
Article Type: Research Article
Received: April 08, 2013
Accepted: August 03, 2013

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Abstract

Background: Recently, the anticancer effect of Chromene derivatives in different cell types has been reported. In this study, we report some derivatives of dihydropyrano[c]chromenes with growth inhibitory and apoptotic activity against K562, Chronic Myeloid Leukaemia (CML).

Methods: The cells were seeded in 96-well plates at 1×105 cells/well and treated with different concentrations of the dihydropyrano[c]chromenes (4-PC, 4-CNC and 4-HC). Growth inhibition and viability determined by trepan blue exclusion test. Apoptosis was also detected by fluorescent microscopy and DNA fragmentation assay.

Results:  The 4-PC, 4-CNC and 4-HC inhibited growth and viability of the cells in dose- and time-dependent manner. These compounds were found to be highly active growth inhibitor with IC50 of 240, 180 and 160 μM, respectively. The results of Acridine orange/ethidium bromide (AO/EtBr) double staining also revealed that the compounds induced apoptosis at respective IC50 value after 72 h of treatment.

Conclusion: As the effect of growth inhibition and induction of apoptosis in K562 Chronic Myeloid Leukaemia cells were observed, dihydropyrano[c]chromenes can be proposed as effective agents for more investigation in leukaemia treatment.

Keywords

Apoptosis Chronic Myeloid Leukaemia dihydropyrano[c]chromenes K562 cell

© 2013, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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