Reconstructing Calvarial Bone Lesions Using PHBV Scaffolds and Cord Blood Mesenchymal Stem Cells in Rat

AUTHORS

Saeed heidari-keshel 1 , Mostafa Rezaei-Tavirani 2 , * , Esmaeil Biazar 3 , Nayb ali Ahmadi 2 , Maryam Ebrahimi 2 , Abdolaziz Ronaghi 2 , Gholamreza Behrozi 2 , Reza Rozafzoon 4

AUTHORS INFORMATION

1 Student Research committee, Proteomics Research Center, Faculty of paramedical sciences, Shahid Beheshti University of medical sciences, Tehran, Iran

2 Proteomics Research Center, Faculty of paramedical sciences, Shahid Beheshti University of medical sciences, Tehran, Iran

3 Department of biomaterial engineering, Tonekabon branch, Islamic azad university, Tonekabon, Iran

4 Stem cell preparation Unit, Eye Research center, Farabi Eye Hospital, Tehran University of medical sciences, Tehran, Iran

How to Cite: heidari-keshel S, Rezaei-Tavirani M, Biazar E, Ahmadi N A, Ebrahimi M, et al. Reconstructing Calvarial Bone Lesions Using PHBV Scaffolds and Cord Blood Mesenchymal Stem Cells in Rat, J Kermanshah Univ Med Sci. 2013 ; 16(8):e77271.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 16 (8); e77271
Published Online: March 29, 2013
Article Type: Research Article
Received: November 26, 2012
Accepted: February 19, 2013

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Abstract

Background: For tissue engineering of bone, anatomical and operational reconstructions of damaged tissue seem to be vital. This is done via reconstruction of bone and appropriate biological joint with bone tissues of damaged areas. In this study the condition of biodegradable bed Nano fiber PHBV and USSC cells were used to accelerate bone repair of damaged area.

Methods: Hollow nanofabrication scaffold of damageable life was designed as PHBV by electrocy and via determining the best factors such as the kind and amount of solvent, specific volume and rate. The separation of osseous tissue infiltration and evaluating its nature by flow cytometrocical analysis was done. Animal test including USSC as well as PHBV condition in the damaged bone was done in the rat. After 8 weeks the implanted area was analyzed using CT-scan and was sent to histopathology ward. Finally, the rate and quality of reconstruction were determined after H and E coloring.

Results: Histomorphic analysis indicated a statistically significant difference between the experimental group of PHBV, USSC+PHBV and control group. Besides, the histopathologic analysis showed that bone reconstruction rate was high in the area containing USSC and PHBV, compared with area having PHBV and control group and consequently the reconstruction quality of bones and the relationship between the new bone tissues and surrounding bone tissues were high too.

Conclusion: Using PHBR scaffold and USSC together could be useful in the amending of wide range of bone lesion.

Keywords

Bone Lesion PHBV stem cells umbilical cord blood

© 2013, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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