The effect of morphine on phagocytosis of peritoneal phagocytes of mice

AUTHORS

Rahil Eftekhari 1 , Hedayatollah Shirzad 1 , Neda Soleimani 1 , Reza Mirzaei 1 , Abolfazl Khoshdel 2 , Mahmoud Rafieian-Kopaei 3 , *

AUTHORS INFORMATION

1 Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

How to Cite: Eftekhari R, Shirzad H, Soleimani N, Mirzaei R, Khoshdel A, et al. The effect of morphine on phagocytosis of peritoneal phagocytes of mice , J Kermanshah Univ Med Sci. 2012 ; 16(4):e78781.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 16 (4); e78781
Published Online: November 29, 2012
Article Type: Research Article
Received: July 24, 2011
Accepted: December 27, 2011

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Abstract

Background: There are reports about the increase of severity of infections in patients receiving morphine. Therefore this study was aimed to evaluate the effect of morphine on phagocytic activity of peritoneal phagocytes of mice.

Methods: Fifty six female Balb/c mice, 6-8 weeks old and 28±2 gram weight, were divided into two groups. The case group received 20, 40, 60 and 80 mg/kg morphine on first, second, third and fourth days, respectively, in the morning and evening, and 100 mg/kg (0.5 cc) once a day on days 5 to 10. Control group received 0.5 cc normal saline at the same time. Then, the mice were killed on days 3, 5 and 10. The peritoneal contents aspirated and mixed with sheep red blood cells (SRBC). Ultimately, the number of phagocytes that engulfed SRBC was determined with light microscope, and compared in two groups.

Results: The number of phagocytes engulfed SRBC in case group was significantly less in the tenth day (the third stage of experiment) in comparison with that of control group (P<0.031).

Conclusion: Prolonged administration of morphine (ten days) decreases the activity of peritoneal phagocytes in Balb/c mice. Therefore, it should be used with caution in patients in whom immune suppression is hazardous or use alternative drugs instead. 

Keywords

phagocytosis mouse macrophage morphine

© 2012, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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