Antioxidant effect of pimpinella anisum and cinnamomum zeylanicum on cell membrane of hepatocytes, LDL, and nonenzymatic glycosylation of hemoglobin

AUTHORS

G Naderi 1 , * , S Asgari 1 , M Taher 1 , M Gharipoor 1 , N Nikkhoo 1

1 Iran

How to Cite: Naderi G, Asgari S, Taher M, Gharipoor M, Nikkhoo N. Antioxidant effect of pimpinella anisum and cinnamomum zeylanicum on cell membrane of hepatocytes, LDL, and nonenzymatic glycosylation of hemoglobin, J Kermanshah Univ Med Sci. 2003 ; 7(1):e81201.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 7 (1); e81201
Published Online: June 19, 2003
Article Type: Research Article
Received: March 15, 2003
Accepted: June 15, 2003

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background & Objectives: Free radicals especially reactive oxygen metabolites can damage DNA, proteins, enzymes, and membrane lipids.  Lipid peroxidation in LDL and hepatocyte membrane may be involved in atherosclerosis and hepatic disease, respectively. nonenzymatic glycosylation of hemoglobin may be involved in complications  of diabetes. Antioxidants including vitamin E may inhibit such reactions. Due to oxidant-antioxidant imbalance, free radicals may cause destructive effects. We studied the antioxidant effects  of pimpinella anisum and cinnamomum zeylanicum on cell membrane of hepatocytes, LDL, and nonenzymatic glycosylation of hemoglobin.

 Materials and Methods: Extracts  of the abovementioned plants were obtained. Rat hepatocyte membrane was selected as a sensitive system to peroxidation. In the vicinity of 1.5mmol of tert-bytyl hydroperoxide as an oxidant agent, and in presence and absence of plant extract, the amount of Malone dialdehyd (MDA), as a marker of lipid peroxidation was measured.  The amount of alanin aminotransferase  as a marker of peroxidation induced cell membrane damage was measured. LDL oxidation and   nonenzymatic glycosylation of hemoglobin were measured in presence and absence of plant extract. T test was used and significance determined at p<0.0.

Results: production of MDA , GOT, and glycosylated hemoglobin was decreased by 10%, ,66% and 16%, respectively by a  dose of 2.5 microgram/ml of Cinnamon. production of MDA , GOT, and glycosylated hemoglobin was decreased by  0.34%, 21%, and 10%,  respectively by a  dose of 2.5 microgram/ml of anisum. 

 Discussion: Cinnamon  had inhibitory effects on all three oxidative systems, and may be useful in preventing atherosclerosis, diabetes and hepatic dysfunction. Anisum had no significant effects on these systems.

Keywords

antioxidants anisum Anisum LDL hepatocytes hemoglobin

© 2003, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Fulltext

The full text of this article is available on PDF

COMMENTS

LEAVE A COMMENT HERE: