Spasmolytic Effect of Vitis Vinifera Leaf Hydroalcoholic Extract on Rat's Isolated Vas Deferens

AUTHORS

MK Gharib-Naseri 1 , * , G Vakilzadeh 1

AUTHORS INFORMATION

1 Iran

How to Cite: Gharib-Naseri M, Vakilzadeh G. Spasmolytic Effect of Vitis Vinifera Leaf Hydroalcoholic Extract on Rat's Isolated Vas Deferens, J Kermanshah Univ Med Sci. 2005 ; 9(1):e81513.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 9 (1); e81513
Published Online: June 19, 2005
Article Type: Research Article
Received: January 10, 2005
Accepted: May 10, 2005

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Abstract

Introduction: Many reports have shown that the grape (Vitis vinifera) seed extract induces antioxidant, hypotensive hypolipidemic, and vasodilatory effects. We have recently shown the relaxatory effect of grape leaf extract on rat's aorta, ileum, uterus and frog's heart rate and contractility. The objective of this study was to investigate the spasmolytic effect of Vitis Vinifera Leaf Hydroalcoholic extract (VLHE) on rat's isolated vas deferens.

Materials & Methods: Vas deferens of male adult rat (Sprague Dawley) was removed, placed in an organ bath containing Tyrode’s solution (30ºC, pH 7.4) and the bath bubbled with air. Then its contractions under 1 g initial tension, were recorded isometrically. The vas deferens was contracted either by KCl (80 mM) or adrenaline (2μg/ml).

 Results: The results demonstrated that the VLHE at 1, 2, 4 and 8 mg/ml reduced the vas deferens contractions evoked by KCl (80 mM) significantly (P<0.0001) and dose-dependently. VLHE (1, 2 and 4 mg/ml) also reduced the adrenaline-induced vas deferens contractions (P<0.0001). In calcium free Tyrod’s solution, KCl-induced contractions were occurred only after adding normal calcium (1.77 mM) to the organ bath solution. VLHE (3mg/ml) reduced the calcium-induced contraction by KCl too. The VLHE-induced vas deferens relaxation was unaffected by the β- adrenoceptor antagonist (propranolol, 3μM for 5 min). Nitric oxide synthase inhibitor (L-NAME, 300μM for 10 min) was unable to modify either KCl-induced contraction or the vas deferens relaxation induced by the extract.

Conclusion: These results suggested that the spasmolytic effect of Vitis vinifera leaf hydroalcoholic extract on rat's vas deferens occurred through the blockage of voltage dependent calcium channels (VDCC). Also, neither NO nor β-adrenoceptors were involved in this inhibitory effect of the extract.

Keywords

Leaf Vitis vinifera Rat Vas Deferens Voltage Dependent Calcium Channels

© 2005, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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