The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

AUTHORS

Hamid Nomani 1 , Ali Aidy 1 , * , Asad Vaisi Raygani 1 , Lida HaghNazari 1 , Dariush Reissi 2 , Hadi Mozafari 1

1 Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Kidney Transplantation Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

How to Cite: Nomani H, Aidy A, Vaisi Raygani A, HaghNazari L, Reissi D, et al. The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease, J Kermanshah Univ Med Sci. 2013 ; 17(8):e74390.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 17 (8); e74390
Published Online: November 29, 2013
Article Type: Research Article
Received: April 13, 2013
Accepted: September 10, 2013

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Abstract

Background: End stage renal disease or ESRD is a progressive and irreversible deterioration in renal function in which the body loses ability to maintain metabolical balance, fluid and electrolytes. Glutathione s-transfrases (GSTs) are members of multigenic family which have essential role as an antioxidant in the cells. In this study, the polymorphism of GST, T1, M1 & P in ESRD patients were compared with those of control groups to determine the possible relationship between polymorphism of these enzymes and ESRD.

Methods: 136 of ESRD patients as experimental and 137 of healthy individuals as control group were examined. The GST T1 & M1 genotypes were determined in all individuals with multiplex- PCR and GST P1 polymorphism was determined with PCR-RFLP.

Results: The distribution of GSTT1& M1 of T1⁺-M1⁺(wild), T1⁺-M1¯, T1¯-M1⁺and T1¯-M1¯(null) genotypes in control group were 45(32.8%), 40(29.2%), 28(20.4%) and 24(17.5%) and in experimental group were17(12.8%), 46(34.6%), 21(15.8%) and 49(36.8%) respectively (P<0.001).

Conclusion: As the reduction of GST T1⁺-M1⁺ (wild) was observed with more frequency in patients with ESRD compared to control group (12.8%vs.32.8%), this genotype can be associated with the risk of ESRD.

Keywords

GST ESRD PCR-RFLP

© 2013, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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