Identification of species of leishmania isolated from patients with cutaneous leishmaniasis in Kermanshah; using RAPD-PCR technique

AUTHORS

Yazdan Hamzavi 1 , * , Bizhan Nomanpour 2 , Ali Gorgin Karaji 3

1 Dept.of Parasitology and Mycology,School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Dept.of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Dept.of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

How to Cite: Hamzavi Y, Nomanpour B, Gorgin Karaji A. Identification of species of leishmania isolated from patients with cutaneous leishmaniasis in Kermanshah; using RAPD-PCR technique, J Kermanshah Univ Med Sci. 2010 ; 14(3):e79482.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 14 (3); e79482
Published Online: December 19, 2010
Article Type: Brief Report
Received: January 10, 2010
Accepted: May 18, 2010

Crossmark

CHEKING

READ FULL TEXT
Abstract

Annually many numbers of pationts with Cutaneous Leishmaniasis (CL) have been reported in Kermanshah province- IRAN. The study aimed to identify species of Leishmania isolated from patients with CT in Kermanshah. Seven isolates of Leishmania obtained from patients with CL, without any travelling to other provinces, were cultured in NNN medium. After mass production of leptomonads in RPMI 1640 medium DNA was purified and the species were diagnosed using RAPD-PCR technique. The study of electrophoretic fingerprints of the product of RAPD-PCR in seven isolates showed that Leshmania major was the causative agent of CL patients in Kermanshah province. More studies in this field recommended.

Keywords

cutaneous leishmaniasis RAPD-PCR Kermanshah

© 2010, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Fulltext

References

  • 1.

    The references of this article is available on PDF

  • COMMENTS

    LEAVE A COMMENT HERE: