Methylation of the GSTP1 gene promotor in colorectal cancer

AUTHORS

Hamid Nomani 1 , * , Naghmeh Jalejo 2 , Bahram Yaghmaei 2 , Shahrokh Mohammadzadeh Ghobadlu 1 , AliAsghar Keshavarz 3

1 Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Dept. of Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Dept. of Pathology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

How to Cite: Nomani H, Jalejo N, Yaghmaei B, Mohammadzadeh Ghobadlu S, Keshavarz A. Methylation of the GSTP1 gene promotor in colorectal cancer, J Kermanshah Univ Med Sci. 2009 ; 13(1):e79827.

ARTICLE INFORMATION

Journal of Kermanshah University of Medical Sciences: 13 (1); e79827
Published Online: June 19, 2009
Article Type: Research Article
Received: December 10, 2008
Accepted: June 09, 2009

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Abstract

Background: Transcriptional silencing of tumor suppressor genes and tumor related genes like GSTP1 by methylation of promotor region CpG island is believed to be an important mechanism in tumorigenesis. The GSTP1 gene encodes the enzyme glutathione S-transferase Pi which defends the cells against oxidative damage and electrophilic carcinogens.

Methods: To gain insight into the role of epigenetic silencing of GSTP1 in colorectal cancer, its methylation was investigated in the blood samples obtained from tumor tissues (n=37) and the adjacent normal tissues (n=29) of patients with colorectal cancer as well as the blood samples taken from control subjects (n=42) by PCR after using methylation – sensitive restriction endonuclease ACCI.

Results: Methylation of GSTP1 was detected in the blood samples of control and blood, adjacent normal tissue and tumor tissue groups at 9.52%, 10.81%, 17.24% and 62.1% in respectively. There was a significant association between methylation of the GSTP1 in tumor tissues and the risk of colorectal cancer compared to adjacent normal tissues (OR=7.86; 95% CI= (2.316-26.633). The difference between methylation of the GSTP1 in the blood samples of case and control grou (OR=1.121; 95% CI = (0.26-4.84) was not statistically significant.

Conclusion: Our findings indicate that methylation of the GSTP1 promotor may be detected in the tumor tissues. This could serve as a molecular diagnosis tool in detection and treatment of colorectal cancer.

Keywords

tumor related gene methylation CpG island glutathione S-transferase Pi colorectal cancer

© 2009, Journal of Kermanshah University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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